Comparative analysis of myometrial and vascular smooth muscle cells to determine optimal cells for use in drug discovery.

ABSTRACT: Novel therapeutic regulators of uterine contractility are needed to manage preterm labor, induce labor and control postpartum hemorrhage. Therefore, we previously developed a high-throughput assay for large-scale screening of small molecular compounds to regulate calcium-mobilization in primary mouse uterine myometrial cells. The goal of this study was to select the optimal myometrial cells for our high-throughput drug discovery assay, as well as determine the similarity or differences of myometrial cells to vascular smooth muscle cells (VSMCs)-the most common off-target of current myometrial therapeutics. Molecular and pharmacological assays were used to compare myometrial cells from four sources: primary cells isolated from term pregnant human and murine myometrium, immortalized pregnant

SUBMITTER: Siricilla S 

PROVIDER: S-EPMC6889064 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature