Integrin alpha-5 subunit is critical for the early stages of human pluripotent stem cell cardiac differentiation.

ABSTRACT: The stem cell niche has a strong influence in the differentiation potential of human pluripotent stem cells with integrins playing a major role in communicating cells with the extracellular environment. However, it is not well understood how interactions between integrins and the extracellular matrix are involved in cardiac stem cell differentiation. To evaluate this, we performed a profile of integrins expression in two stages of cardiac differentiation: mesodermal progenitors and cardiomyocytes. We found an active regulation of the expression of different integrins during cardiac differentiation. In particular, integrin α5 subunit showed an increased expression in mesodermal progenitors, and a significant downregulation in cardiomyocytes. To analyze the effect of α5 subunit, we modified

SUBMITTER: Neiman G 

PROVIDER: S-EPMC6889169 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature