Label-Free Assay of Protein Tyrosine Phosphatase Activity in Single Cells.

ABSTRACT: Populations of cells exhibit variations in biochemical activity, resulting from many factors including random stochastic variability in protein production, metabolic and cell-cycle states, regulatory mechanisms, and external signaling. The development of methods for the analysis of single cells has allowed for the measurement and understanding of this inherent heterogeneity, yet methods for measuring protein activities on the single-cell scale lag behind their genetic analysis counterparts and typically report on expression rather than activity. This paper presents an approach to measure protein tyrosine phosphatase (PTP) activity in individual cells using self-assembled monolayers for matrix-assisted laser desorption/ionization mass spectrometry. Using flow cytometry, individual cells are

SUBMITTER: Moully EH 

PROVIDER: S-EPMC6889211 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature