Plug-and-Play In Vitro Metastasis System toward Recapitulating the Metastatic Cascade.

ABSTRACT: Microfluidic-based tumor models that mimic tumor culture environment have been developed to understand the cancer metastasis mechanism and discover effective antimetastatic drugs. These models successfully recapitulated key steps of metastatic cascades, yet still limited to few metastatic steps, operation difficulty, and small molecule absorption. In this study, we developed a metastasis system made of biocompatible and drug resistance plastics to recapitulate each metastasis stage in three-dimensional (3D) mono- and co-cultures formats, enabling the investigation of the metastatic responses of cancer cells (A549-GFP). The plug-and-play feature enhances the efficiency of the experimental setup and avoids initial culture failures. The results demonstrate that cancer cells tended to prolifer

SUBMITTER: Ni BS 

PROVIDER: S-EPMC6889311 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature