Evaluating tools for transcription factor binding site prediction.

ABSTRACT:

Background

Binding of transcription factors to transcription factor binding sites (TFBSs) is key to the mediation of transcriptional regulation. Information on experimentally validated functional TFBSs is limited and consequently there is a need for accurate prediction of TFBSs for gene annotation and in applications such as evaluating the effects of single nucleotide variations in causing disease. TFBSs are generally recognized by scanning a position weight matrix (PWM) against DNA using one of a number of available computer programs. Thus we set out to evaluate the best tools that can be used locally (and are therefore suitable for large-scale analyses) for creating PWMs from high-throughput ChIP-Seq data and for scanning them against DNA.

Results

We evaluated a set of de

SUBMITTER: Jayaram N 

PROVIDER: S-EPMC6889335 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature