Dynamic methylome of internal mRNA N⁷-methylguanosine and its regulatory role in translation.

ABSTRACT: Over 150 types of RNA modifications are identified in RNA molecules. Transcriptome profiling is one of the key steps in decoding the epitranscriptomic panorama of these chemical modifications and their potential functions. N⁷-methylguanosine (m⁷G) is one of the most abundant modifications present in tRNA, rRNA and mRNA 5'cap, and has critical roles in regulating RNA processing, metabolism and function. Besides its presence at the cap position in mRNAs, m⁷G is also identified in internal mRNA regions. However, its transcriptome-wide distribution and dynamic regulation within internal mRNA regions remain unknown. Here, we have established m⁷G individual-nucleotide-resolution cross-linking and immunoprecipitation with sequencing (m⁷G miCL

SUBMITTER: Malbec L 

PROVIDER: S-EPMC6889513 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature