Chromosome level assembly and comparative genome analysis confirm lager-brewing yeasts originated from a single hybridization.

ABSTRACT:

Background

The lager brewing yeast, S. pastorianus, is a hybrid between S. cerevisiae and S. eubayanus with extensive chromosome aneuploidy. S. pastorianus is subdivided into Group 1 and Group 2 strains, where Group 2 strains have higher copy number and a larger degree of heterozygosity for S. cerevisiae chromosomes. As a result, Group 2 strains were hypothesized to have emerged from a hybridization event distinct from Group 1 strains. Current genome assemblies of S. pastorianus strains are incomplete and highly fragmented, limiting our ability to investigate their evolutionary history.

Results

To fill this gap, we generated a chromosome-level genome assembly of the S. pastorianus strain CBS 1483 from Oxford Nanopore MinION DNA sequencing data and analysed the newly assemble

SUBMITTER: Salazar AN 

PROVIDER: S-EPMC6889557 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature