Early detection of the major male cancer types in blood-based liquid biopsies using a DNA methylation panel.

ABSTRACT:

Background

Lung (LC), prostate (PCa) and colorectal (CRC) cancers are the most incident in males worldwide. Despite recent advances, optimal population-based cancer screening methods remain an unmet need. Due to its early onset, cancer specificity and accessibility in body fluids, aberrant DNA promoter methylation might be a valuable minimally invasive tool for early cancer detection. Herein, we aimed to develop a minimally invasive methylation-based test for simultaneous early detection of LC, PCa and CRC in males, using liquid biopsies.

Results

Circulating cell-free DNA was extracted from 102 LC, 121 PCa and 100 CRC patients and 136 asymptomatic donors' plasma samples. Sodium-bisulfite modification and whole-genome amplification was performed. Promoter methylation levels o

SUBMITTER: Constancio V 

PROVIDER: S-EPMC6889617 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature