Microglial activation by microbial neuraminidase through TLR2 and TLR4 receptors.

ABSTRACT:

Background

Neuraminidase (NA) is a sialidase present, among various locations, in the envelope/membrane of some bacteria/viruses (e.g., influenza virus), and is involved in infectiveness and/or dispersion. The administration of NA within the brain lateral ventricle represents a model of acute sterile inflammation. The relevance of the Toll-like receptors TLR2 and TLR4 (particularly those in microglial cells) in such process was investigated.

Methods

Mouse strains deficient in either TLR2 (TLR2^-/-) or TLR4 (TLR4^-/-) were used. NA was injected in the lateral ventricle, and the inflammatory reaction was studied by immunohistochemistry (IBA1 and IL-1β) and qPCR (cytokine response). Also, microglia was isolated from those strains and in vitro stimulated with

SUBMITTER: Fernandez-Arjona MDM 

PROVIDER: S-EPMC6889729 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature