Drosophila PSI controls circadian period and the phase of circadian behavior under temperature cycle via tim splicing.

ABSTRACT: The Drosophila circadian pacemaker consists of transcriptional feedback loops subjected to post-transcriptional and post-translational regulation. While post-translational regulatory mechanisms have been studied in detail, much less is known about circadian post-transcriptional control. Thus, we targeted 364 RNA binding and RNA associated proteins with RNA interference. Among the 43 hits we identified was the alternative splicing regulator P-element somatic inhibitor (PSI). PSI regulates the thermosensitive alternative splicing of timeless (tim), promoting splicing events favored at warm temperature over those increased at cold temperature. Psi downregulation shortens the period of circadian rhythms and advances the phase of circadian behavior under temperature

SUBMITTER: Foley LE 

PROVIDER: S-EPMC6890465 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature