Analysing the rice young panicle transcriptome reveals the gene regulatory network controlled by TRIANGULAR HULL1.

ABSTRACT:

Background

TRIANGULAR HULL1 (TH1), a member of the rice ALOG gene family, has been characterized as a rice lemma/palea-related gene. To understand the gene regulatory network that controlled by TH1, we analyzed the transcriptome from a TH1 knock out (KO) line, which was generated by CRISPR/Cas9. Our study may shed some light on the molecular mechanism of lemma/palea development.

Results

We obtained 20 T₀ th1-C transgenic plants by CRISPR/Cas9. Among the 20 plants, there were eight bi-allelic mutations, five homozygous mutations, three heterozygous mutations, and four Non-KO plants. By comparing with the wild type and the heterozygous knock out (KO) line, the homozygous KO lines showed defects in lemma/palea development as well as in grain filling. Further more, we

SUBMITTER: Wang J 

PROVIDER: S-EPMC6890884 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature