Interferon-Responsive Genes Are Targeted during the Establishment of Human Cytomegalovirus Latency.

ABSTRACT: Human cytomegalovirus (HCMV) latency is an active process which remodels the latently infected cell to optimize latent carriage and reactivation. This is achieved, in part, through the expression of viral genes, including the G-protein-coupled receptor US28. Here, we use an unbiased proteomic screen to assess changes in host proteins induced by US28, revealing that interferon-inducible genes are downregulated by US28. We validate that major histocompatibility complex (MHC) class II and two pyrin and HIN domain (PYHIN) proteins, myeloid cell nuclear differentiation antigen (MNDA) and IFI16, are downregulated during experimental latency in primary human CD14⁺ monocytes. We find that IFI16 is targeted rapidly during the establishment of latency in a US28-dependent manner but only i

SUBMITTER: Elder EG 

PROVIDER: S-EPMC6890990 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature