Rapid and Differential Evolution of the Venom Composition of a Parasitoid Wasp Depending on the Host Strain.

ABSTRACT: Parasitoid wasps rely primarily on venom to suppress the immune response and regulate the physiology of their host. Intraspecific variability of venom protein composition has been documented in some species, but its evolutionary potential is poorly understood. We performed an experimental evolution initiated with the crosses of two lines of Leptopilinaboulardi of different venom composition to generate variability and create new combinations of venom factors. The offspring were maintained for 10 generations on two strains of Drosophila melanogaster differing in resistance/susceptibility to the parental parasitoid lines. The venom composition of individuals was characterized by a semi-automatic analysis of 1D SDS-PAGE electrophoresis protein profiles whose accuracy was checked by Western bl

SUBMITTER: Cavigliasso F 

PROVIDER: S-EPMC6891688 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature