Development and application of an antibody detection ELISA for Haemophilus parasuis based on a monomeric autotransporter passenger domain.

ABSTRACT:

Background

Haemophilus parasuis is a commensal pathogen in the swine upper respiratory tract and causes Glässer's disease. Surveillance, screening for infection, and vaccination response of H. parasuis is hindered by the lack of a rapid antibody detection method.

Results

In the present study, a monomeric autotransporter was identified as a novel antigen for developing an indirect ELISA. The autotransporter passenger domain (Apd) was expressed, purified, and demonstrated to be specific in ELISA and western blotting. Mouse antiserum of recombinant Apd (rApd) recognized native Apd in the 15 serotype reference strains and five non-typeable isolate stains, but showed no reaction with seven other bacterial pathogens. The rApd ELISA was optimized and validated using 67 serum sample

SUBMITTER: Liu Y 

PROVIDER: S-EPMC6891974 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature