Potential factors contributing to the poor antimicrobial efficacy of SAAP-148 in a rat wound infection model.

ABSTRACT:

Background

We investigated the efficacy of a synthetic antimicrobial peptide SAAP-148, which was shown to be effective against Methicillin-resistant Staphylococcus aureus (MRSA) on tape-stripped mice skin. Unexpectedly, SAAP-148 was not effective against MRSA in our pilot study using rats with excision wounds. Therefore, we investigated factors that might have contributed to the poor efficacy of SAAP-148. Subsequently, we optimised the protocol and assessed the efficacy of SAAP-148 in an adapted rat study.

Methods

We incubated 100 µL of SAAP-148 with 1 cm² of a wound dressing for 1 h and determined the unabsorbed volume of peptide solution. Furthermore, 10⁵ colony forming units (CFU)/mL MRSA were exposed to increasing dosages of SAAP-148 in 50% (v/v) hu

SUBMITTER: Dijksteel GS 

PROVIDER: S-EPMC6891976 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature