Comparing dental plaque microbiome diversity of extrinsic black stain in the primary dentition using Illumina MiSeq sequencing technique.

ABSTRACT:

Background

Extrinsic black stain (EBS) is characterized by discrete dark dots or lines on the tooth surface. The relationship between EBS and oral microbiota in children remains elusive. The aim of this study was to compare dental plaque microbiome in EBS children with that in EBS-free children in the primary dentition.

Methods

The Illumina MiSeq sequencing technique was utilized in the cross-sectional pilot study to investigate the diversity and composition of the supragingival plaque microbiota from 10 EBS-positive and 10 EBS-free children. The results were analysed with nonparametric Mann-Whitney U test, Pearson Chi-Square test, Fisher's Exact test and one-way ANOVA tests.

Results

We identified 13 different phyla, 22 classes, 33 orders, 54 families, 105 genera, and

SUBMITTER: Chen L 

PROVIDER: S-EPMC6892020 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature