Active pyruvate dehydrogenase and impaired gluconeogenesis in orthotopic hepatomas of rats.

ABSTRACT: BACKGROUND:Therapies targeting altered activity of pyruvate dehydrogenase (PDH) and pyruvate carboxylase (PC) have been proposed for hepatomas. However, the activities of these pathways in hepatomas in vivo have not been distinguished. Here we examined pyruvate entry into the tricarboxylic acid (TCA) cycle through PDH versus PC in vivo using hepatoma-bearing rats. METHODS:Hepatoma-bearing rats were generated by intrahepatic injection of H4IIE cells. Metabolism of ¹³C-labeled glycerol, a physiological substrate for both gluconeogenesis and energy production, was measured with ¹³C NMR analysis. The concentration of key metabolites and the expression of relevant enzymes were measured in hepatoma, surrounding liver, and normal liver. RESULTS:In orthotopic hepatomas, pyruv

SUBMITTER: Lee MH 

PROVIDER: S-EPMC6892165 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature