Chaperone activity of serine protease HtrA of Helicobacter pylori as a crucial survival factor under stress conditions.

ABSTRACT:

Background

Serine protease HtrA exhibits both proteolytic and chaperone activities, which are involved in cellular protein quality control. Moreover, HtrA is an important virulence factor in many pathogens including Helicobacter pylori, for which the crucial stage of infection is the cleavage of E-cadherin and other cell-to-cell junction proteins.

Methods

The in vitro study of H. pylori HtrA (HtrA_Hp) chaperone activity was carried out using light scattering assays and investigation of lysozyme protein aggregates. We produced H. pylori ∆htrA deletion and HtrA_Hp point mutants without proteolytic activity in strain N6 and investigated the survival of the bacteria under thermal, osmotic, acidic and general stress conditions as well as the presence of puromy

SUBMITTER: Zarzecka U 

PROVIDER: S-EPMC6892219 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature