A model for determining cardiac mitochondrial substrate utilisation using stable ¹³C-labelled metabolites.

ABSTRACT:

Introduction

Relative oxidation of different metabolic substrates in the heart varies both physiologically and pathologically, in order to meet metabolic demands under different circumstances. ¹³C labelled substrates have become a key tool for studying substrate use-yet an accurate model is required to analyse the complex data produced as these substrates become incorporated into the Krebs cycle.

Objectives

We aimed to generate a network model for the quantitative analysis of Krebs cycle intermediate isotopologue distributions measured by mass spectrometry, to determine the ¹³C labelled proportion of acetyl-CoA entering the Krebs cycle.

Methods

A model was generated, and validated ex vivo using isotopic distributions measured from isolated hearts

SUBMITTER: Lindsay RT 

PROVIDER: S-EPMC6892366 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature