Genome-wide analysis of the H3K27me3 epigenome and transcriptome in Brassica rapa.

ABSTRACT:

Background

Genome-wide maps of histone modifications have been obtained for several plant species. However, most studies focus on model systems and do not enforce FAIR data management principles. Here we study the H3K27me3 epigenome and associated transcriptome of Brassica rapa, an important vegetable cultivated worldwide.

Findings

We performed H3K27me3 chromatin immunoprecipitation followed by high-throughput sequencing and transcriptomic analysis by 3'-end RNA sequencing from B. rapa leaves and inflorescences. To analyze these data we developed a Reproducible Epigenomic Analysis pipeline using Galaxy and Jupyter, packaged into Docker images to facilitate transparency and reuse. We found that H3K27me3 covers roughly one-third of all B. rapa protein-coding genes and its pres

SUBMITTER: Paya-Milans M 

PROVIDER: S-EPMC6892454 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature