Laminin-driven Epac/Rap1 regulation of epithelial barriers on decellularized matrix.

ABSTRACT: Decellularized tissues offer a unique tool for developing regenerative biomaterials or in vitro platforms for the study of cell-extracellular matrix (ECM) interactions. One main challenge associated with decellularized lung tissue is that ECM components can be stripped away or altered by the detergents used to remove cellular debris. Without characterizing the composition of lung decellularized ECM (dECM) and the cellular response caused by the altered composition, it is difficult to utilize dECM for regeneration and specifically, engineering the complexities of the alveolar-capillary barrier. This study takes steps towards uncovering if dECM must be enhanced with lost ECM proteins to achieve proper epithelial barrier formation. To achieve this, the epithelial barrier function was assessed

SUBMITTER: Young BM 

PROVIDER: S-EPMC6892605 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature