Photo-activated raster scanning thermal imaging at sub-diffraction resolution.

ABSTRACT: Active thermal imaging is a valuable tool for the nondestructive characterization of the morphological properties and the functional state of biological tissues and synthetic materials. However, state-of-the-art techniques do not typically combine the required high spatial resolution over extended fields of view with the quantification of temperature variations. Here, we demonstrate quantitative far-infrared photo-thermal imaging at sub-diffraction resolution over millimeter-sized fields of view. Our approach combines the sample absorption of modulated raster-scanned laser light with the automated localization of the laser-induced temperature variations imaged by a thermal camera. With temperature increments ∼0.5-5 °C, we achieve a six-time gain with respect to our 350-μm diffraction-limit

SUBMITTER: Bouzin M 

PROVIDER: S-EPMC6892803 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature