Circadian rhythms in Per1, PER2 and Ca²⁺ of a solitary SCN neuron cultured on a microisland.

ABSTRACT: Circadian rhythms in Per1, PER2 expression and intracellular Ca²⁺ were measured from a solitary SCN neuron or glial cell which was physically isolated from other cells. Dispersed cells were cultured on a platform of microisland (100-200 μm in diameter) in a culture dish. Significant circadian rhythms were detected in 57.1% for Per1 and 70.0% for PER2 expression. When two neurons were located on the same island, the circadian rhythms showed desynchronization, indicating a lack of oscillatory coupling. Circadian rhythms were also detected in intracellular Ca²⁺ of solitary SCN neurons. The ratio of circadian positive neurons was significantly larger without co-habitant of glial cells (84.4%) than with it (25.0%). A relatively large fraction of SCN neurons generates the i

SUBMITTER: Hirata Y 

PROVIDER: S-EPMC6892917 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature