Polyphosphate, Zn²⁺ and high molecular weight kininogen modulate individual reactions of the contact pathway of blood clotting.

ABSTRACT:

Background

Inorganic polyphosphate modulates the contact pathway of blood clotting, which is implicated in thrombosis and inflammation. Polyphosphate polymer lengths are highly variable, with shorter polymers (approximately 60-100 phosphates) secreted from human platelets, and longer polymers (up to thousands of phosphates) in microbes. We previously reported that optimal triggering of clotting via the contact pathway requires very long polyphosphates, although the impact of shorter polyphosphate polymers on individual proteolytic reactions of the contact pathway was not interrogated.

Objectives and methods

We conducted in vitro measurements of enzyme kinetics to investigate the ability of varying polyphosphate sizes, together with high molecular weight kininogen and Zn

SUBMITTER: Wang Y 

PROVIDER: S-EPMC6893101 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature