A Toxicity Screening Approach to Identify Bacteriophage-Encoded Anti-Microbial Proteins.

ABSTRACT: The rapid emergence of antibiotic resistance among many pathogenic bacteria has created a profound need to discover new alternatives to antibiotics. Bacteriophages, the viruses of microbes, express special proteins to overtake the metabolism of the bacterial host they infect, the best known of which are involved in bacterial lysis. However, the functions of majority of bacteriophage encoded gene products are not known, i.e., they represent the hypothetical proteins of unknown function (HPUFs). In the current study we present a phage genomics-based screening approach to identify phage HPUFs with antibacterial activity with a long-term goal to use them as leads to find unknown targets to develop novel antibacterial compounds. The screening assay is based on the inhibition of bacterial growth

SUBMITTER: Mohanraj U 

PROVIDER: S-EPMC6893735 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature