Analysis of Swine Conventional Dendritic Cells, DEC205⁺CD172a^+/-CADM1⁺, from Blood and Spleen in Response to PRRSV and PEDV.

ABSTRACT: Conventional dendritic cells (cDCs) cannot be infected by porcine reproductive and respiratory syndrome virus (PRRSV) but respond to infection via cytokine production, indicating a possible role in initiation/regulation of the immune response against PRRSV. In this work, we evaluated the responses of splenic and blood cDCs, with DEC205⁺CADM1⁺CD172a^+/- phenotype, as well as those of CD163⁺ cells against PRRSV and porcine epidemic diarrhea virus (PEDV). Both populations were incubated in the presence of PRRSV or PEDV with and without naïve CD3⁺ T cells, and cytokine responses were evaluated by qPCR and ELISA. Our results showed that cDCs, but not CD163⁺ cells, produced IL-12 in response to PRRSV. PEDV did not induce IL-12 prod

SUBMITTER: Parra-Sanchez H 

PROVIDER: S-EPMC6893809 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature