Forster Resonance Energy Transfer-Mediated Globular Protein Sensing Using Polyelectrolyte Complex Nanoparticles.

ABSTRACT: Polyelectrolyte complex nanoparticles (PEC NPs) are synthesized using two oppositely charged polyelectrolytes, i.e., anionic poly(sodium 4-styrene sulfonate) (PSS) and cationic poly(diallyldimethylammoniumchloride), at molar mixing ratios (n -/n +) of ≈0.4, 0.67, 0.75, and 1.5 by applying consecutive centrifugation to modify the optical property of PSS. However, for n -/n + ≈ 0.75, PEC NPs exhibit a larger blue shift and a specific emission peak occurs at ≈278 nm for the 225 nm excitation. The mechanism of such modification of PSS emission after complex formation is proposed. This specific emission by PEC NPs nearly matches with the optical absorption wavelength of globular proteins. The emission intensity of PEC NPs is therefore quenched in the presence of globular proteins (bovine serum

SUBMITTER: Talukdar H 

PROVIDER: S-EPMC6893948 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature