Human Cerebral Organoids and Fetal Brain Tissue Share Proteomic Similarities.

ABSTRACT: The limited access to functional human brain tissue has led to the development of stem cell-based alternative models. The differentiation of human pluripotent stem cells into cerebral organoids with self-organized architecture has created novel opportunities to study the early stages of the human cerebral formation. Here we applied state-of-the-art label-free shotgun proteomics to compare the proteome of stem cell-derived cerebral organoids to the human fetal brain. We identified 3,073 proteins associated with different developmental stages, from neural progenitors to neurons, astrocytes, or oligodendrocytes. The major protein groups are associated with neurogenesis, axon guidance, synaptogenesis, and cortical brain development. Glial cell proteins related to cell growth and maintenance, e

SUBMITTER: Nascimento JM 

PROVIDER: S-EPMC6893972 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature