Spatiotemporal analysis of impaired microglia process movement at sites of secondary neurodegeneration post-stroke.

ABSTRACT: It has recently been identified that after motor cortex stroke, the ability of microglia processes to respond to local damage cues is lost from the thalamus, a major site of secondary neurodegeneration (SND). In this study, we combine a photothrombotic stroke model in mice, acute slice and fluorescent imaging to analyse the loss of microglia process responsiveness. The peri-infarct territories and thalamic areas of SND were investigated at time-points 3, 7, 14, 28 and 56 days after stroke. We confirmed the highly specific nature of non-responsive microglia processes to sites of SND. Non-responsiveness was at no time observed at the peri-infarct but started in the thalamus seven days post-stroke and persisted for 56 days. Loss of directed process extension is not a reflection of general fun

SUBMITTER: Kluge MG 

PROVIDER: S-EPMC6893987 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature