Transcriptional profiling of human intestinal plasma cells reveals effector functions beyond antibody production.

ABSTRACT:

Background

Plasma cells (PCs) are terminally differentiated B-lymphocytes producing antibodies. In coeliac disease (CeD) there is increased density of PCs in the small-intestinal lesion. Many of these PCs produce disease-specific autoantibodies targeting transglutaminase 2 (TG2).

Objective

The plasmacytosis of CeD motivated us to study the transcriptional programme of PCs from coeliac gut lesions.

Methods

RNA-seq was performed on the PCs of CeD patients and disease controls, being specific or non-specific for TG2.

Results

Being antibody-producing cells, 67% of the PCs' transcript was aligned to immunoglobulin genes. Strikingly, genes encoding ligands and receptors of chemokines and cytokines were abundant. Higher transcript levels of genes associated with cell

SUBMITTER: Snir O 

PROVIDER: S-EPMC6893993 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature