A microfluidic alternating-pull-push active digitization method for sample-loss-free digital PCR.

ABSTRACT: Digital polymerase chain reaction (dPCR) is a powerful tool for genetic analysis, providing superior sensitivity and accuracy. In many applications that demand minuscule reaction volumes, such as single cell analysis, efficient and reproducible sample handling and digitization is pivotal for accurate absolute quantification of targets, but remains a significant technical challenge. In this paper, we described a robust and flexible microfluidic alternating-pull-push active digitization (μAPPAD) strategy that confers close to 100% sample digitization efficiency for microwell-based dPCR. Our strategy employs pneumatic valve control to periodically manipulate air pressure inside the chip to greatly facilitate the vacuum-driven partition of solution into microwells, enabling efficient digitizat

SUBMITTER: Zhou X 

PROVIDER: S-EPMC6894176 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature