Improving selectivity of DNA-RNA binding zinc finger using directed evolution.

ABSTRACT:

Objective

Type C2H2 zinc fingers bind a variety of substrates, specific sequences in the double-stranded DNA counting among them. Engineering efforts led to the discovery of a set of general rules that enable obtaining zinc fingers modules that bind to almost any given sequence. The objective of this work was to determine an analogical set of rules for the binding of specific sequences in DNA-RNA hybrids using directed evolution of ZfQQR zinc finger. The target regions for evolution included the amino acid residues that directly interact with the substrate and linkers between the zinc finger modules.

Results

The directed evolution was performed using selection based on biopanning of phage-displayed libraries of randomized regions in the ZfQQR zinc finger. The applied strateg

SUBMITTER: Sulej AA 

PROVIDER: S-EPMC6894256 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature