Multiplex ligation reaction based on probe melting curve analysis: a pragmatic approach for the identification of 30 common Salmonella serovars.

ABSTRACT: BACKGROUND:While Salmonella serotyping is of paramount importance for the disease intervention of salmonellosis, a fast and easy-to-operate molecular serotyping solution remains elusive. We have developed a multiplex ligation reaction based on probe melting curve analysis (MLMA) for the identification of 30 common Salmonella serovars. METHODS:Serovar-specific primers and probes were designed based on a comparison of gene targets (wzx and wzy encoding for somatic antigen biosynthesis; fliC and fljB for flagellar antigens) from 5868 Salmonella genomes. The ssaR gene, a type III secretion system component, was included for the confirmation of Salmonella. RESULTS:All gene targets were detected and gave expected Tm values during assay evaluation. Cross reactions were not demonstrated between th

SUBMITTER: Zuo L 

PROVIDER: S-EPMC6894471 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature