Target genes associated with lipid and glucose metabolism in non-alcoholic fatty liver disease.

ABSTRACT: BACKGROUND:Insulin resistance (IR) and lipid peroxidation are accepted as 'two-hit' hypothesis of Non-alcoholic fatty liver disease (NAFLD). However, there are few published research on identifying genes which connect lipid and glucose metabolism by gene microarray. OBJECTIVE:To identify target genes related to lipid and glucose metabolism that might be responsible for the pathogenesis of NAFLD. METHODS:A rat model of NAFLD was established by feeding male rats with high-fat diet and gene expression profiles of liver tissues were determined using Agilent DNA microarray. We then investigated differentially expressed genes (DEGs) and intersection of them by using Gene Ontology (GO) and Pathway Analyses. Target genes were verified by Real-time polymerase chain reaction (RT-PCR). RESULTS:Compar

SUBMITTER: Li T 

PROVIDER: S-EPMC6894500 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature