High-throughput microcircuit analysis of individual human brains through next-generation multineuron patch-clamp.

ABSTRACT: Comparing neuronal microcircuits across different brain regions, species and individuals can reveal common and divergent principles of network computation. Simultaneous patch-clamp recordings from multiple neurons offer the highest temporal and subthreshold resolution to analyse local synaptic connectivity. However, its establishment is technically complex and the experimental performance is limited by high failure rates, long experimental times and small sample sizes. We introduce an in vitro multipatch setup with an automated pipette pressure and cleaning system facilitating recordings of up to 10 neurons simultaneously and sequential patching of additional neurons. We present hardware and software solutions that increase the usability, speed and data throughput of multipatch experiments

SUBMITTER: Peng Y 

PROVIDER: S-EPMC6894931 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature