Profilin-Mediated Actin Allocation Regulates the Growth of Epithelial Microvilli.

ABSTRACT: Transporting epithelial cells, like those that line the intestinal tract, are specialized for solute processing and uptake. One defining feature is the brush border, an array of microvilli that serves to amplify apical membrane surface area and increase functional capacity. During differentiation, upon exit from stem-cell-containing crypts, enterocytes build thousands of microvilli, each supported by a parallel bundle of actin filaments several microns in length. Given the high concentration of actin residing in mature brush borders, we sought to determine whether enterocytes were resource (i.e., actin monomer) limited in assembling this domain. To examine this possibility, we inhibited Arp2/3, the ubiquitous branched actin nucleator, to increase G-actin availability during brush border as

SUBMITTER: Faust JJ 

PROVIDER: S-EPMC6894984 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature