Small RNA ArcZ Regulates Oxidative Stress Response Genes and Regulons in Erwinia amylovora.

ABSTRACT: Erwinia amylovora, causative agent of fire blight disease of apple and pear trees, has evolved to use small RNAs for post-transcriptional regulation of virulence traits important for disease development. The sRNA ArcZ regulates several virulence traits, and to better understand its roles, we conducted a transcriptomic comparison of wild-type and ΔarcZ mutant E. amylovora. We found that ArcZ regulates multiple cellular processes including genes encoding enzymes involved in mitigating the threat of reactive oxygen species (katA, tpx, osmC), and that the ΔarcZ mutant has reduced catalase activity and is more susceptible to exogenous hydrogen peroxide. We quantified hydrogen peroxide production by apple leaves inoculated with E. amylovora

SUBMITTER: Schachterle JK 

PROVIDER: S-EPMC6895013 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature