Deconvolution of nucleic-acid length distributions: a gel electrophoresis analysis tool and applications.

ABSTRACT: Next-generation DNA-sequencing (NGS) technologies, which are designed to streamline the acquisition of massive amounts of sequencing data, are nonetheless dependent on various preparative steps to generate DNA fragments of required concentration, purity and average size (molecular weight). Current automated electrophoresis systems for DNA- and RNA-sample quality control, such as Agilent's Bioanalyzer® and TapeStation® products, are costly to acquire and use; they also provide limited information for samples having broad size distributions. Here, we describe a software tool that helps determine the size distribution of DNA fragments in an NGS library, or other DNA sample, based on gel-electrophoretic line profiles. The software, developed as an ImageJ plug-in, allows for straightforward pro

SUBMITTER: Ziraldo R 

PROVIDER: S-EPMC6895257 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature