Super-resolution microscopy reveals how histone tail acetylation affects DNA compaction within nucleosomes in vivo.

ABSTRACT: Chromatin organization is crucial for regulating gene expression. Previously, we showed that nucleosomes form groups, termed clutches. Clutch size correlated with the pluripotency grade of mouse embryonic stem cells and human induced pluripotent stem cells. Recently, it was also shown that regions of the chromatin containing activating epigenetic marks were composed of small and dispersed chromatin nanodomains with lower DNA density compared to the larger silenced domains. Overall, these results suggest that clutch size may regulate DNA packing density and gene activity. To directly test this model, we carried out 3D, two-color super-resolution microscopy of histones and DNA with and without increased histone tail acetylation. Our results showed that lower percentage of DNA was associated

SUBMITTER: Otterstrom J 

PROVIDER: S-EPMC6895258 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature