Engineering DNA recognition and allosteric response properties of TetR family proteins by using a module-swapping strategy.

ABSTRACT: The development of synthetic biological systems requires modular biomolecular components to flexibly alter response pathways. In previous studies, we have established a module-swapping design principle to engineer allosteric response and DNA recognition properties among regulators in the LacI family, in which the engineered regulators served as effective components for implementing new cellular behavior. Here we introduced this protein engineering strategy to two regulators in the TetR family: TetR (UniProt Accession ID: P04483) and MphR (Q9EVJ6). The TetR DNA-binding module and the MphR ligand-binding module were used to create the TetR-MphR. This resulting hybrid regulator possesses DNA-binding properties of TetR and ligand response properties of MphR, which is able to control gene expre

SUBMITTER: Dimas RP 

PROVIDER: S-EPMC6895282 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature