Chromatin structure dynamics during the mitosis-to-G1 phase transition.

ABSTRACT: Features of higher-order chromatin organization-such as A/B compartments, topologically associating domains and chromatin loops-are temporarily disrupted during mitosis^1,2. Because these structures are thought to influence gene regulation, it is important to understand how they are re-established after mitosis. Here we examine the dynamics of chromosome reorganization by Hi-C after mitosis in highly purified, synchronous mouse erythroid cell populations. We observed rapid establishment of A/B compartments, followed by their gradual intensification and expansion. Contact domains form from the 'bottom up'-smaller subTADs are formed initially, followed by convergence into multi-domain TAD structures. CTCF is partially retained on mitotic chromosomes and immediately resumes full bin

SUBMITTER: Zhang H 

PROVIDER: S-EPMC6895436 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature