NMR Reveals Light-Induced Changes in the Dynamics of a Photoswitchable Fluorescent Protein.

ABSTRACT: The availability of fluorescent proteins with distinct phototransformation properties is crucial for a wide range of applications in advanced fluorescence microscopy and biotechnology. To rationally design new variants optimized for specific applications, a detailed understanding of the mechanistic features underlying phototransformation is essential. At present, little is known about the conformational dynamics of fluorescent proteins at physiological temperature and how these dynamics contribute to the observed phototransformation properties. Here, we apply high-resolution NMR spectroscopy in solution combined with in situ sample illumination at different wavelengths to investigate the conformational dynamics of rsFolder, a GFP-derived protein that can be reversibly switched between a gr

SUBMITTER: Christou NE 

PROVIDER: S-EPMC6895687 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature