Confinement-Free Wide-Field Ratiometric Tracking of Single Fluorescent Molecules.

ABSTRACT: Single-molecule fluorescence has been highly instrumental in elucidating interactions and dynamics of biological molecules in the past two decades. Single-molecule fluorescence experiments usually rely on one of two detection geometries, either confocal point-detection or wide-field area detection, typically in a total internal reflection fluorescence (TIRF) format. However, each of these techniques suffers from fundamental drawbacks that limit their application. In this work, we present a new technique, solution wide-field imaging (SWiFi) of diffusing molecules, as an alternative to the existing methods. SWiFi is a simple extension to existing objective-type TIRF microscopes that allows wide-field observations of fast-diffusing molecules down to single fluorophores without the need of tet

SUBMITTER: Gilboa B 

PROVIDER: S-EPMC6895709 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature