Synergism Through WEE1 and CHK1 Inhibition in Acute Lymphoblastic Leukemia.

ABSTRACT:

Introduction

Screening for synthetic lethality markers has demonstrated that the inhibition of the cell cycle checkpoint kinases WEE1 together with CHK1 drastically affects stability of the cell cycle and induces cell death in rapidly proliferating cells. Exploiting this finding for a possible therapeutic approach has showed efficacy in various solid and hematologic tumors, though not specifically tested in acute lymphoblastic leukemia.

Methods

The efficacy of the combination between WEE1 and CHK1 inhibitors in B and T cell precursor acute lymphoblastic leukemia (B/T-ALL) was evaluated in vitro and ex vivo studies. The efficacy of the therapeutic strategy was tested in terms of cytotoxicity, induction of apoptosis, and changes in cell cycle profile and protein expression usi

SUBMITTER: Ghelli Luserna Di Rora A 

PROVIDER: S-EPMC6895917 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature