Efficient Gene Disruption via Base Editing Induced Stop in Newt Pleurodeles waltl.

ABSTRACT: Loss-of-function approaches provide strong evidence for determining the role of particular genes. The prevalent CRISPR/Cas9 technique is widely used to disrupt target gene with uncontrolled non-homologous end joining after the double strand breaks, which results in mosaicism and multiple genotypes in the founders. In animal models with long generation time such as the salamanders, producing homozygous offspring mutants would be rather labor intensive and time consuming. Here we utilized the base editing technique to create the loss-of-function F0 mutants without the random indels. As a proof of principle, we successfully introduced premature stop codons into the tyrosinase locus and produced the albino phenotype in the newts (Pleurodeles waltl). We further demonstrated that the knockout ef

SUBMITTER: Cai H 

PROVIDER: S-EPMC6895984 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature