Identification of an Embryonic Cell-Specific Region within the Pineapple SERK1 Promoter.

ABSTRACT: Plant tissue culture methods, such as somatic embryogenesis, are attractive alternatives to traditional breeding methods for plant propagation. However, they often suffer from limited efficiency. Somatic embryogenesis receptor kinase (SERK)1 is a marker gene of early somatic embryogenesis in several plants, including pineapple. It can be selectively induced and promotes a key step in somatic embryogenesis. We investigated the embryonic cell-specific transcriptional regulation of AcSERK1 by constructing a series of vectors carrying the GUS（Beta-glucuronidase） reporter gene under the control of different candidate cis-regulatory sequences. These vectors were transfected into both embryonic and non-embryonic callus, and three immature embryo stages and the embryonic-specific activity of the p

SUBMITTER: Luan A 

PROVIDER: S-EPMC6896011 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature