Cost-effective high-throughput single-haplotype iterative mapping and sequencing for complex genomic structures.

ABSTRACT: The reference sequences of structurally complex regions can be obtained only through highly accurate clone-based approaches. We and others have successfully used single-haplotype iterative mapping and sequencing (SHIMS) 1.0 to assemble structurally complex regions across the sex chromosomes of several vertebrate species and to allow for targeted improvements to the reference sequences of human autosomes. However, SHIMS 1.0 is expensive and time consuming, requiring resources that only a genome center can provide. Here we introduce SHIMS 2.0, an improved SHIMS protocol that allows even a small laboratory to generate high-quality reference sequence from complex genomic regions. Using a streamlined and parallelized library-preparation protocol, and taking advantage of inexpensive high-through

SUBMITTER: Bellott DW 

PROVIDER: S-EPMC6896216 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature