A gene regulatory network controls the balance between mesendoderm and ectoderm at pluripotency exit.

ABSTRACT: During embryogenesis, differentiation of pluripotent cells into somatic cell types depends both on signaling cues and intrinsic gene expression programs. While the molecular underpinnings of pluripotency are well mapped, much less is known on how mouse embryonic stem cells (mESCs) differentiate. Using RNA-Seq profiling during specification to the three germ layers, we showed that mESCs switched on condition-specific gene expression programs from the onset of the differentiation procedure and that primed pluripotency did not constitute an obligatory intermediate state. After inferring the gene network controlling mESC differentiation, we tested the role of the highly connected nodes by deleting them in a triple knock-in Sox1-Brachyury-Eomes mESC line reporting on ectoderm, mesoderm, and end

SUBMITTER: Sladitschek HL 

PROVIDER: S-EPMC6896232 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature