PCR-based reverse genetics strategy for bluetongue virus recovery.

ABSTRACT:

Background

Bluetongue virus (BTV), an emerging insect vector mediated pathogen affecting both wild ruminants and livestock, has a genome consisting of 10 linear double-stranded RNA genome segments. BTV has a severe economic impact on agriculture in many parts of the world. Current reverse genetics (RG) strategy to rescue BTV mainly rely on in vitro synthesis of RNA transcripts from cloned complimentary DNA (cDNA) corresponding to viral genome segments with the aid of helper plasmids. RNA synthesis is a laborious job which is further complicated with a need for expensive reagents and a meticulous operational procedure. Additionally, the target genes must be cloned into a specific vector to prepare templates for RNA transcription.

Result

In this study, we have developed a PCR

SUBMITTER: Xu Q 

PROVIDER: S-EPMC6896262 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature