Cytokine production and phenotype of Histomonas meleagridis-specific T cells in the chicken.

ABSTRACT: The protozoan parasite Histomonas meleagridis is the causative agent of the re-emerging disease histomonosis of chickens and turkeys. Due to the parasite's extracellular occurrence, a type-2 differentiation of H. meleagridis-specific T cells has been hypothesized. In contrast, a recent study suggested that IFN-γ mRNA⁺ cells are involved in protection against histomonosis. However, the phenotype and cytokine production profile of H. meleagridis-specific T cells still awaits elucidation. In this work, clonal cultures of a virulent monoxenic strain of H. meleagridis were used for infecting chickens to detect IFN-γ protein and IL-13 mRNA by intracellular cytokine staining and PrimeFlow™ RNA Assays, respectively, in CD4⁺ and CD8β⁺ T cells. Infection was confirme

SUBMITTER: Lagler J 

PROVIDER: S-EPMC6896354 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature